Hotspot

rps11-rpl36

LSC · 84454-84617 · 164 bp

Back to Gagnebina

Pi 0.0124

MarkerSeek score 61.40

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 164
variable_sites: 2
pi: 0.0124
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TACGCGCATTCTTACGTGAA	CGTAGACGCGGACGAATTAT	45.0	50.0	58.0	58.0	294-297 bp	1.0	81.0
rps11-rpl36_p2	TTACGCGCATTCTTACGTGA	CGTAGACGCGGACGAATTAT	45.0	50.0	58.0	58.0	295-298 bp	1.0	81.0
rps11-rpl36_p3	TACGCGCATTCTTACGTGAA	AAAATAAGGGCCTCCGTTCG	45.0	50.0	58.0	58.3	342-345 bp	1.0	79.0
rps11-rpl36_p4	TTACGCGCATTCTTACGTGA	AAAATAAGGGCCTCCGTTCG	45.0	50.0	58.0	58.3	343-346 bp	1.0	79.0
rps11-rpl36_p5	TACGCGCATTCTTACGTGAA	ATAAGGGCCTCCGTTCGTAA	45.0	50.0	58.0	58.5	339-342 bp	1.0	76.9