Hotspot

petA-psbJ

LSC · 67303-68180 · 878 bp

Back to Gagnebina

Pi 0.00458

MarkerSeek score 61.80

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 878
variable_sites: 4
pi: 0.00458
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GTCTCTTGTTCTTCGTGGCA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.1	57.6	1002-1005 bp	1.0	76.6
petA-psbJ_p2	CGTGTCCAAGGTCTCTTGTT	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.8	57.6	1012-1015 bp	1.0	75.8
petA-psbJ_p3	TGTTCTTCGTGGCATCTGTT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	996-999 bp	1.0	75.7
petA-psbJ_p4	TTGTTCTTCGTGGCATCTGT	ATTCCGGATTGGGTTCATCC	45.0	50.0	57.7	57.6	997-1000 bp	1.0	75.7
petA-psbJ_p5	CGTTACGTGTCCAAGGTCTC	ATTCCGGATTGGGTTCATCC	55.0	50.0	58.3	57.6	1017-1020 bp	1.0	75.3