Hotspot

psbZ-trnG-GCC

LSC · 40043-40631 · 589 bp

Back to Gagnebina

Pi 0.00515

MarkerSeek score 54.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 589
variable_sites: 3
pi: 0.00515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 54.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTTCTCCTGATGGTTGGTCG	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.0	57.7	744-776 bp	1.0	78.3
psbZ-trnG-GCC_p2	TTGCTTCTCCTGATGGTTGG	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	747-779 bp	1.0	76.8
psbZ-trnG-GCC_p3	TCCTGATGGTTGGTCGAGTA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	57.8	57.7	740-772 bp	1.0	76.7
psbZ-trnG-GCC_p4	GCTTCTCCTGATGGTTGGTC	GCGTCTTCTCTTTGGCAAAG	55.0	50.0	58.3	57.7	745-777 bp	1.0	76.4
psbZ-trnG-GCC_p5	TTCTCCTGATGGTTGGTCGA	GCGTCTTCTCTTTGGCAAAG	50.0	50.0	58.4	57.7	743-775 bp	1.0	75.6