Hotspot

psbC-trnS-UGA

LSC · 39190-39432 · 243 bp

Back to Gagnebina

Pi 0.0422
MarkerSeek score 79.00
Primer available yes
Label psbC-trnS-UGA

Feature Metadata

label_name
psbC-trnS-UGA
feature_type
igs
region
LSC
length_bp
243
variable_sites
10
pi
0.0422
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbC-trnS-UGA_p1 TCATTTATGGCACGCAGGAA GAGAGATGGCTGAGTGGTTG 45.0 55.0 57.9 58.0 432-438 bp 1.0 81.8
psbC-trnS-UGA_p2 TCATTTATGGCACGCAGGAA GCTGAGTGGTTGATAGCTCC 45.0 55.0 57.9 58.1 424-430 bp 1.0 81.6
psbC-trnS-UGA_p3 TCATTTATGGCACGCAGGAA GGCTGAGTGGTTGATAGCTC 45.0 55.0 57.9 58.1 425-431 bp 1.0 81.6
psbC-trnS-UGA_p4 TCATTTATGGCACGCAGGAA GGTTGATAGCTCCGGTCTTG 45.0 55.0 57.9 58.1 417-423 bp 1.0 81.6
psbC-trnS-UGA_p5 TTTATGGCACGCAGGAAGAG GAGAGATGGCTGAGTGGTTG 50.0 55.0 58.3 58.0 429-435 bp 1.0 80.7