Hotspot

atpH-atpI

LSC · 15196-16732 · 1537 bp

Back to Gagnebina

Pi 0.00523

MarkerSeek score 62.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1537
variable_sites: 8
pi: 0.00523
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	AGCGGCAGAAATCAATGGAT	AGTGGTTCCGATACCTGTCA	45.0	50.0	57.9	58.1	1676-1684 bp	1.0	79.7
atpH-atpI_p2	AGCGGCAGAAATCAATGGAT	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.9	57.9	1616-1624 bp	1.0	79.2
atpH-atpI_p3	AAGCGGCAGAAATCAATGGA	AGTGGTTCCGATACCTGTCA	45.0	50.0	58.2	58.1	1677-1685 bp	1.0	78.9
atpH-atpI_p4	CGAAGCGGCAGAAATCAATG	AGTGGTTCCGATACCTGTCA	50.0	50.0	58.2	58.1	1679-1687 bp	1.0	78.8
atpH-atpI_p5	GAAGCGGCAGAAATCAATGG	AGTGGTTCCGATACCTGTCA	50.0	50.0	57.8	58.1	1678-1686 bp	1.0	78.6