Hotspot

trnR-UCU-atpA

LSC · 11036-11408 · 373 bp

Back to Gagnebina

Pi 0.0164

MarkerSeek score 60.70

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 373
variable_sites: 6
pi: 0.0164
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	AAGACATTCACTCGGGAAGC	47.6	50.0	57.3	57.9	483-491 bp	1.0	68.0
trnR-UCU-atpA_p2	GGTTCAAATCCTATTGGACGC	CCAAGACATTCACTCGGGAA	47.6	50.0	57.3	57.5	485-493 bp	1.0	65.0
trnR-UCU-atpA_p3	TGGATAGGACAGAGGTCTTCT	AAGACATTCACTCGGGAAGC	47.6	50.0	56.9	57.9	518-526 bp	1.0	64.9
trnR-UCU-atpA_p4	GGTTCAAATCCTATTGGACGC	ACCAAGACATTCACTCGGGA	47.6	50.0	57.3	58.7	486-494 bp	1.0	63.5
trnR-UCU-atpA_p5	GTTCAAATCCTATTGGACGCA	AAGACATTCACTCGGGAAGC	42.9	50.0	56.6	57.9	482-490 bp	1.0	62.3