Hotspot

rps16-trnQ-UUG

LSC · 6653-7275 · 623 bp

Back to Gagnebina

Pi 0.00818

MarkerSeek score 65.30

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 623
variable_sites: 5
pi: 0.00818
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	GTCTTTCAAGTCGCACGTTG	TCGAATAGAAATGGGGCGTG	50.0	50.0	58.0	58.1	751-762 bp	1.0	81.3
rps16-trnQ-UUG_p2	GTCTTTCAAGTCGCACGTTG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.0	58.1	692-703 bp	1.0	81.3
rps16-trnQ-UUG_p3	ATGTCTTTCAAGTCGCACGT	TCGAATAGAAATGGGGCGTG	45.0	50.0	58.1	58.1	753-764 bp	1.0	80.3
rps16-trnQ-UUG_p4	ATGTCTTTCAAGTCGCACGT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	694-705 bp	1.0	80.3
rps16-trnQ-UUG_p5	GCACGTTGCTTTTTACCACA	TCGAATAGAAATGGGGCGTG	45.0	50.0	57.8	58.1	739-750 bp	1.0	79.8