Hotspot

psaC-ndhE

SSC · 113027-113445 · 419 bp

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Pi 0.0534
MarkerSeek score 78.60
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
419
variable_sites
38
pi
0.0534
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 ACATCCGTCCCAAGGTATCA CTTTGGACCCTTGGAGATCG 50.0 55.0 58.1 58.0 1250-1281 bp 1.0 84.9
psaC-ndhE_p2 GAACCCGCGAATATAGGCAA CTTTGGACCCTTGGAGATCG 50.0 55.0 58.1 58.0 1579-1610 bp 1.0 83.9
psaC-ndhE_p3 CTGGCCAAGCTGCTAAAGTA CTTTGGACCCTTGGAGATCG 50.0 55.0 57.9 58.0 1842-1873 bp 1.0 83.8
psaC-ndhE_p4 TGGCCAAGCTGCTAAAGTAG CTTTGGACCCTTGGAGATCG 50.0 55.0 57.9 58.0 1841-1872 bp 1.0 83.8
psaC-ndhE_p5 AAATAGGTCCCACGGAAAGC CTTTGGACCCTTGGAGATCG 50.0 55.0 57.9 58.0 1794-1825 bp 0.75 73.1