Hotspot

rpl22-rps19

LSC · 80457-80525 · 69 bp

Back to Gagea

Pi 0.0507
MarkerSeek score 78.50
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
69
variable_sites
7
pi
0.0507
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 TTTCTGTACTCCAACGCGAG GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 722-725 bp 1.0 82.8
rpl22-rps19_p2 CGTGCAATTCCTTCGAAACC GTTATCCTGCGCTTGGAAGA 50.0 50.0 58.0 58.0 1265-1274 bp 1.0 82.2
rpl22-rps19_p3 AAACGTACCCGCATTGACTT GTTATCCTGCGCTTGGAAGA 45.0 50.0 58.1 58.0 964-973 bp 1.0 81.9
rpl22-rps19_p4 CGTGCAATTCCTTCGAAACC GATCCACTTGGCTACATCCG 50.0 55.0 58.0 58.1 1084-1097 bp 1.0 81.8
rpl22-rps19_p5 TTCGAAACCAGAGGACGTTC GTTATCCTGCGCTTGGAAGA 50.0 50.0 57.9 58.0 1254-1263 bp 1.0 81.3