Hotspot

clpP-psbB

LSC · 68636-69081 · 446 bp

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Pi 0.0463
MarkerSeek score 86.20
Primer available yes
Label clpP-psbB

Feature Metadata

label_name
clpP-psbB
feature_type
igs
region
LSC
length_bp
446
variable_sites
41
pi
0.0463
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP-psbB_p1 CGCACTATACGTCAACCCAA CCCAACCAGAAACTAGAGCC 50.0 55.0 58.0 57.9 602-647 bp 1.0 88.4
clpP-psbB_p2 CGCACTATACGTCAACCCAA GGCCCAACCAGAAACTAGAG 50.0 55.0 58.0 57.9 604-649 bp 1.0 88.4
clpP-psbB_p3 CGCACTATACGTCAACCCAA CGATACCAAGGCAAACCCAT 50.0 50.0 58.0 58.2 522-567 bp 1.0 88.2
clpP-psbB_p4 CGCACTATACGTCAACCCAA CCAACCAGAAACTAGAGCCG 50.0 55.0 58.0 58.3 601-646 bp 1.0 87.1
clpP-psbB_p5 ACCCAAACTGCATCTTCCTC CCCAACCAGAAACTAGAGCC 50.0 55.0 57.8 57.9 588-633 bp 1.0 86.8