Hotspot

petA-psbJ

LSC · 59895-60498 · 604 bp

Back to Gagea

Pi 0.0453
MarkerSeek score 77.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
604
variable_sites
52
pi
0.0453
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 AGAAACAGTTTGAAAAGGTTCA GGTTCATATTCCGGGTTGGG 31.8 55.0 54.6 58.3 681-722 bp 1.0 47.8
petA-psbJ_p2 AGAAACAGTTTGAAAAGGTTCA CATATTCCGGGTTGGGTTCA 31.8 50.0 54.6 57.6 677-718 bp 1.0 47.8
petA-psbJ_p3 AGAAACAGTTTGAAAAGGTTCA GTTCATATTCCGGGTTGGGT 31.8 50.0 54.6 57.6 680-721 bp 1.0 47.8
petA-psbJ_p4 AGAAACAGTTTGAAAAGGTTCA GCTGGTATTCCTGTGATCGG 31.8 55.0 54.6 58.1 723-764 bp 1.0 47.3
petA-psbJ_p5 AAGAAACAGTTTGAAAAGGTTCA GCTGGTATTCCTGTGATCGG 30.4 55.0 55.4 58.1 724-765 bp 1.0 47.3