Hotspot

rbcL-accD

LSC · 53569-54357 · 789 bp

Back to Gagea

Pi 0.0423
MarkerSeek score 78.60
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
789
variable_sites
59
pi
0.0423
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 CGTGAAGCTTGCAAATGGAG GTCATCCGAGACCAAACTCC 50.0 55.0 58.0 58.0 1101-1128 bp 0.5 66.6
rbcL-accD_p2 TGCCTGCTCTAACCGAAATC GTCATCCGAGACCAAACTCC 50.0 55.0 58.0 58.0 1277-1304 bp 0.5 65.7
rbcL-accD_p3 GGAGAACGCGAGATGACTTT GTCATCCGAGACCAAACTCC 50.0 55.0 58.0 58.0 1428-1455 bp 0.5 65.3
rbcL-accD_p4 TTGAATGCTACTGCGGGTAC GTCATCCGAGACCAAACTCC 50.0 55.0 58.0 58.0 1719-1746 bp 0.5 64.5
rbcL-accD_p5 GGCTCATTATTGCCGAGACA GTCATCCGAGACCAAACTCC 50.0 55.0 58.0 58.0 1597-1624 bp 0.5 64.5