Hotspot

trnL_UAA-trnF_GAA

LSC · 45437-45631 · 195 bp

Back to Gagea

Pi 0.0538
MarkerSeek score 80.50
Primer available yes
Label trnL_UAA-trnF_GAA

Feature Metadata

label_name
trnL_UAA-trnF_GAA
feature_type
igs
region
LSC
length_bp
195
variable_sites
19
pi
0.0538
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL_UAA-trnF_GAA_p1 AAGTCGTGAGGGTTCAAGTC TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 270-307 bp 1.0 84.6
trnL_UAA-trnF_GAA_p2 GAAGTCGTGAGGGTTCAAGT TTTGAACTGGTGACACGAGG 50.0 50.0 57.5 58.1 271-308 bp 1.0 84.6
trnL_UAA-trnF_GAA_p3 AGAAGTCGTGAGGGTTCAAG TTTGAACTGGTGACACGAGG 50.0 50.0 57.2 58.1 272-309 bp 1.0 82.2
trnL_UAA-trnF_GAA_p4 AAGTCGTGAGGGTTCAAGTC TACCAACTGAGCTATCCCGA 50.0 50.0 57.5 57.6 232-269 bp 1.0 82.2
trnL_UAA-trnF_GAA_p5 GAAGTCGTGAGGGTTCAAGT TACCAACTGAGCTATCCCGA 50.0 50.0 57.5 57.6 233-270 bp 1.0 82.2