Hotspot

trnE_UUC-trnT_GGU

LSC · 29561-30172 · 612 bp

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Pi 0.0496
MarkerSeek score 84.50
Primer available yes
Label trnE_UUC-trnT_GGU

Feature Metadata

label_name
trnE_UUC-trnT_GGU
feature_type
igs
region
LSC
length_bp
612
variable_sites
58
pi
0.0496
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE_UUC-trnT_GGU_p1 CCCAGCAAGATGTATGGCAT GAACCGATGACTTACGCCTT 50.0 50.0 58.0 58.0 1169-1249 bp 1.0 87.3
trnE_UUC-trnT_GGU_p2 GGATCCTAGTTCGGGACTGA GAACCGATGACTTACGCCTT 55.0 50.0 57.9 58.0 1251-1331 bp 1.0 86.3
trnE_UUC-trnT_GGU_p3 CCCAGCAAGATGTATGGCAT TGGGTCAGATCCACTTACGT 50.0 50.0 58.0 58.1 1401-1492 bp 1.0 86.3
trnE_UUC-trnT_GGU_p4 CCCAGCAAGATGTATGGCAT CCATGGCGTTACTCTACCAC 50.0 55.0 58.0 58.1 1148-1228 bp 0.75 74.7
trnE_UUC-trnT_GGU_p5 CTTGCTCCCTGTCCCATTTT GAACCGATGACTTACGCCTT 50.0 50.0 58.1 58.0 1307 bp 0.25 49.4