Hotspot

trnS_GCU-trnG_UCC

LSC · 7656-8540 · 885 bp

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Pi 0.0503
MarkerSeek score 86.20
Primer available yes
Label trnS_GCU-trnG_UCC

Feature Metadata

label_name
trnS_GCU-trnG_UCC
feature_type
igs
region
LSC
length_bp
885
variable_sites
81
pi
0.0503
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS_GCU-trnG_UCC_p1 ATTAGCAATCCGCCGCTTTA ACGAATCACACTTTTACCACT 45.0 38.1 58.0 55.5 938-994 bp 1.0 62.1
trnS_GCU-trnG_UCC_p2 ATTAGCAATCCGCCGCTTTA AGAACGAATCACACTTTTACCAC 45.0 39.1 58.0 57.3 941-997 bp 1.0 61.0
trnS_GCU-trnG_UCC_p3 ATTAGCAATCCGCCGCTTTA GAACGAATCACACTTTTACCACT 45.0 39.1 58.0 57.3 940-996 bp 1.0 61.0
trnS_GCU-trnG_UCC_p4 ATTAGCAATCCGCCGCTTTA AGGGGTTAATAGAACGAATCACA 45.0 39.1 58.0 57.3 951-1007 bp 1.0 60.8
trnS_GCU-trnG_UCC_p5 ATTAGCAATCCGCCGCTTTA GGGGTTAATAGAACGAATCACAC 45.0 43.5 58.0 57.3 950-1006 bp 1.0 60.5