Hotspot

psbK-psbI

LSC · 7211-7313 · 103 bp

Back to Gagea

Pi 0.0599
MarkerSeek score 80.70
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
103
variable_sites
11
pi
0.0599
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
80.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GCAGGCTGCTGTAAGTTTTC ACGAAGAGTTTGAGAGTAAGCA 50.0 40.9 57.7 57.4 150-156 bp 1.0 66.4
psbK-psbI_p2 AGGCTGCTGTAAGTTTTCGA ACGAAGAGTTTGAGAGTAAGCA 45.0 40.9 57.5 57.4 148-154 bp 1.0 64.8
psbK-psbI_p3 CAGGCTGCTGTAAGTTTTCG ACGAAGAGTTTGAGAGTAAGCA 50.0 40.9 57.4 57.4 149-155 bp 1.0 64.3
psbK-psbI_p4 GCAGGCTGCTGTAAGTTTTC AACGAAGAGTTTGAGAGTAAGCA 50.0 39.1 57.7 58.1 151-157 bp 1.0 62.6
psbK-psbI_p5 GCAGGCTGCTGTAAGTTTTC ACGAAGAGTTTGAGAGTAAGCAT 50.0 39.1 57.7 57.9 150-156 bp 1.0 61.9