Hotspot

rps16-trnQ_UUG

LSC · 5599-6612 · 1014 bp

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Pi 0.0578
MarkerSeek score 87.10
Primer available yes
Label rps16-trnQ_UUG

Feature Metadata

label_name
rps16-trnQ_UUG
feature_type
igs
region
LSC
length_bp
1014
variable_sites
104
pi
0.0578
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.97
markerseek_score
87.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ_UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 992-1123 bp 1.0 90.0
rps16-trnQ_UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1003-1134 bp 1.0 89.4
rps16-trnQ_UUG_p3 AAGTCGCACGTTGCTTTCTA CTCGGAGGTTCGAATCCTTC 45.0 55.0 58.1 57.8 996-1127 bp 1.0 88.9
rps16-trnQ_UUG_p4 TCATGTCCTTCAAGTCGCAC CTCGGAGGTTCGAATCCTTC 50.0 55.0 58.2 57.8 1007-1138 bp 1.0 88.3
rps16-trnQ_UUG_p5 AAGTCGCACGTTGCTTTCTA TACTCGGAGGTTCGAATCCT 45.0 50.0 58.1 57.6 998-1129 bp 1.0 87.1