Hotspot

trnK-UUU-rps16

LSC · 4049-4480 · 432 bp

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Pi 0.0494
MarkerSeek score 86.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
432
variable_sites
39
pi
0.0494
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.98
markerseek_score
86.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 AAAGCCGAGTACTCTACCGT AAAATGGCGCTCAACCTACA 50.0 45.0 58.2 58.1 529-565 bp 1.0 87.6
trnK-UUU-rps16_p2 AAGCCGAGTACTCTACCGTT AAAATGGCGCTCAACCTACA 50.0 45.0 58.2 58.1 528-564 bp 1.0 87.6
trnK-UUU-rps16_p3 AAAGCCGAGTACTCTACCGT TGAAAATGGCGCTCAACCTA 50.0 45.0 58.2 57.8 531-567 bp 1.0 86.8
trnK-UUU-rps16_p4 AAGCCGAGTACTCTACCGTT TGAAAATGGCGCTCAACCTA 50.0 45.0 58.2 57.8 530-566 bp 1.0 86.8
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT CTTGAAAATGGCGCTCAACC 50.0 50.0 58.2 58.3 532-568 bp 1.0 85.9