Hotspot

rps3

SSC · 120720-121388 · 669 bp

Pi 0.00598

MarkerSeek score 62.20

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: SSC
length_bp: 669
variable_sites: 4
pi: 0.00598
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	GGCTTGAATTGCATAGTCACC	AATCCGTAAGCCGACTTGTC	47.6	50.0	57.6	58.0	872 bp	1.0	68.9
rps3_p2	GGGCTTGAATTGCATAGTCAC	AATCCGTAAGCCGACTTGTC	47.6	50.0	57.6	58.0	873 bp	1.0	68.9
rps3_p3	TCGTTCTTTTGGGACTAAGCA	AATCCGTAAGCCGACTTGTC	42.9	50.0	57.5	58.0	786 bp	1.0	68.5
rps3_p4	GGCTTGAATTGCATAGTCACC	TAGATTTCGACCACGTGCTC	47.6	50.0	57.6	57.7	908 bp	1.0	66.7
rps3_p5	GGGCTTGAATTGCATAGTCAC	TAGATTTCGACCACGTGCTC	47.6	50.0	57.6	57.7	909 bp	1.0	66.7