Hotspot

rpl22

SSC · 121421-121753 · 333 bp

Pi 0.003

MarkerSeek score 40.30

Primer available yes

Label rpl22

Feature Metadata

label_name: rpl22
feature_type: gene
region: SSC
length_bp: 333
variable_sites: 1
pi: 0.003
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22_p1	CGACCTGGTACCACAGATTG	TCCTTGTGATCATCCGCATG	55.0	50.0	58.0	58.0	1142 bp	1.0	80.2
rpl22_p2	ACACGACCTTCTCTTACCCA	TCCTTGTGATCATCCGCATG	50.0	50.0	58.0	58.0	1442 bp	1.0	80.1
rpl22_p3	CTTACCCATTCTGTCCGAGC	TCCTTGTGATCATCCGCATG	55.0	50.0	58.1	58.0	1430 bp	1.0	79.7
rpl22_p4	ACACGACCTTCTCTTACCCA	GAAGGTCGTGCAACAATTGG	50.0	50.0	58.0	57.9	1414 bp	0.5	59.6
rpl22_p5	CGACCTGGTACCACAGATTG	GAAGGTCGTGCAACAATTGG	55.0	50.0	58.0	57.9	1114 bp	0.5	59.5