Hotspot

chlB

LSC · 26180-27712 · 1533 bp

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Pi 0.00261
MarkerSeek score 52.40
Primer available yes
Label chlB

Feature Metadata

label_name
chlB
feature_type
gene
region
LSC
length_bp
1533
variable_sites
4
pi
0.00261
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
chlB_p1 TGAGTATTTGAATTTGATACTCTCA GCTACGTCCCGTAAGTCATT 28.0 50.0 54.3 57.4 1672 bp 1.0 40.2
chlB_p2 TTGAGTATTTGAATTTGATACTCTCA GCTACGTCCCGTAAGTCATT 26.9 50.0 55.0 57.4 1673 bp 1.0 40.2
chlB_p3 TGAGTATTTGAATTTGATACTCTCA CTACCAAACTGCGCTACGTC 28.0 55.0 54.3 59.0 1684 bp 1.0 40.2
chlB_p4 TGAGTATTTGAATTTGATACTCTCAT GCTACGTCCCGTAAGTCATT 26.9 50.0 54.8 57.4 1672 bp 1.0 40.2
chlB_p5 TGAGTATTTGAATTTGATACTCTCA TACCAAACTGCGCTACGTC 28.0 52.6 54.3 57.9 1683 bp 1.0 40.2