Hotspot

atpF

LSC · 41593-42117 · 525 bp

Back to Fucus

Pi 0.00571
MarkerSeek score 60.50
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
525
variable_sites
3
pi
0.00571
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 TCTCAATGAAACAACCGCCA ACTTGTTTCCAACTGTTTTGCT 45.0 36.4 58.0 57.5 1333 bp 1.0 59.9
atpF_p2 GGCTAGTTGTGGCATTAGCT ACTTGTTTCCAACTGTTTTGCT 50.0 36.4 58.0 57.5 1849 bp 1.0 59.7
atpF_p3 TGGGCTAGTTGTGGCATTAG ACTTGTTTCCAACTGTTTTGCT 50.0 36.4 57.6 57.5 1851 bp 1.0 56.7
atpF_p4 TCTCAATGAAACAACCGCCA ACTTGTTTCCAACTGTTTTGC 45.0 38.1 58.0 56.0 1333 bp 1.0 56.2
atpF_p5 GGCTAGTTGTGGCATTAGCT ACTTGTTTCCAACTGTTTTGC 50.0 38.1 58.0 56.0 1849 bp 1.0 56.0