Hotspot

trnH(gtg)-trnT(tgt)

SSC · 96740-96789 · 50 bp

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Pi 0.02
MarkerSeek score 64.00
Primer available yes
Label trnH(gtg)-trnT(tgt)

Feature Metadata

label_name
trnH(gtg)-trnT(tgt)
feature_type
igs
region
SSC
length_bp
50
variable_sites
1
pi
0.02
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(gtg)-trnT(tgt)_p1 GGTTCAAGTCCCGTCATTCA AGCTGGTGAAAGGACTTGAA 50.0 45.0 57.8 57.0 145 bp 1.0 70.9
trnH(gtg)-trnT(tgt)_p2 TTCAAGTCCCGTCATTCACC AGCTGGTGAAAGGACTTGAA 50.0 45.0 57.8 57.0 143 bp 1.0 70.9
trnH(gtg)-trnT(tgt)_p3 GGTTCAAGTCCCGTCATTCA GCTGGTGAAAGGACTTGAAC 50.0 50.0 57.8 56.9 144 bp 1.0 70.6
trnH(gtg)-trnT(tgt)_p4 TTCAAGTCCCGTCATTCACC GCTGGTGAAAGGACTTGAAC 50.0 50.0 57.8 56.9 142 bp 1.0 70.6
trnH(gtg)-trnT(tgt)_p5 GTTATAAGGTGGGTGTGGCC AGCTGGTGAAAGGACTTGAA 55.0 45.0 58.2 57.0 204 bp 1.0 70.2