Hotspot

ORF76-trnY(gta)

LSC · 25882-25915 · 34 bp

Back to Fucus

Pi 0.0882
MarkerSeek score 78.50
Primer available yes
Label ORF76-trnY(gta)

Feature Metadata

label_name
ORF76-trnY(gta)
feature_type
igs
region
LSC
length_bp
34
variable_sites
3
pi
0.0882
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ORF76-trnY(gta)_p1 TGTTTGGTGGGATAGAGGGA AAGGGGGCGGATTGTAAATC 50.0 50.0 57.6 57.9 137 bp 1.0 78.4
ORF76-trnY(gta)_p2 TGTTTGGTGGGATAGAGGGA GCTGGTTTACCTACGTTGGT 50.0 50.0 57.6 57.8 116 bp 1.0 77.9
ORF76-trnY(gta)_p3 TGTTTGGTGGGATAGAGGGA AATCCGCTGGTTTACCTACG 50.0 50.0 57.6 57.7 121 bp 1.0 76.7
ORF76-trnY(gta)_p4 TGTTTGGTGGGATAGAGGGA GTTCAAATCCAACTCGGCCT 50.0 50.0 57.6 58.5 98 bp 1.0 75.9
ORF76-trnY(gta)_p5 TGTTTGGTGGGATAGAGGGA GTTAAAGGGGGCGGATTGTA 50.0 50.0 57.6 57.6 141 bp 1.0 75.5