Hotspot

ycf46-ccs1

LSC · 23833-23866 · 34 bp

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Pi 0.0294
MarkerSeek score 69.40
Primer available yes
Label ycf46-ccs1

Feature Metadata

label_name
ycf46-ccs1
feature_type
igs
region
LSC
length_bp
34
variable_sites
1
pi
0.0294
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf46-ccs1_p1 ACAAAATTGGGCTTCTTCAGG TGCTCAATAAATGAACCAATACT 42.9 30.4 57.0 54.3 175 bp 1.0 40.5
ycf46-ccs1_p2 ACAAAATTGGGCTTCTTCAGG TTTGCTCAATAAATGAACCAA 42.9 28.6 57.0 52.2 177 bp 1.0 40.5
ycf46-ccs1_p3 ACAAAATTGGGCTTCTTCAGG TTGCTCAATAAATGAACCAATACT 42.9 29.2 57.0 55.1 176 bp 1.0 40.5
ycf46-ccs1_p4 ACAAAATTGGGCTTCTTCAGG TTTTGCTCAATAAATGAACCA 42.9 28.6 57.0 52.2 178 bp 1.0 40.4
ycf46-ccs1_p5 ACAAAATTGGGCTTCTTCAGG TTTTGCTCAATAAATGAACCAA 42.9 27.3 57.0 53.1 178 bp 1.0 40.4