Hotspot

ycf1-2

Genome · 104007-105416 · 1410 bp

Back to Frullania

Pi 0.0184

MarkerSeek score 56.10

Primer available yes

Label ycf1-2

Feature Metadata

label_name: ycf1-2
feature_type: gene
region: Genome
length_bp: 1410
variable_sites: 26
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-2_p1	CCACAGACCTCGTTGAGATG	ACTGCCTGGGGTTTCTTAAC	55.0	50.0	58.0	57.7	2411-2426 bp	1.0	80.1
ycf1-2_p2	TCCACAGACCTCGTTGAGAT	ACTGCCTGGGGTTTCTTAAC	50.0	50.0	58.1	57.7	2412-2427 bp	1.0	79.5
ycf1-2_p3	CCACAGACCTCGTTGAGATG	TGCCTGGGGTTTCTTAACTG	55.0	50.0	58.0	57.7	2424 bp	0.5	57.7
ycf1-2_p4	TCCACAGACCTCGTTGAGAT	TGCCTGGGGTTTCTTAACTG	50.0	50.0	58.1	57.7	2425 bp	0.5	57.1
ycf1-2_p5	GCCGTAAAACACCATGCATC	TGCCTGGGGTTTCTTAACTG	50.0	50.0	58.1	57.7	2654 bp	0.5	57.0