Hotspot

ycf1-1

Genome · 105486-108641 · 3156 bp

Back to Frullania

Pi 0.0171

MarkerSeek score 51.40

Primer available yes

Label ycf1-1

Feature Metadata

label_name: ycf1-1
feature_type: gene
region: Genome
length_bp: 3156
variable_sites: 54
pi: 0.0171
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-1_p1	ACTGGTTTTTCTATTTTCGCT	TAACGTTTCGTGCTAGTCCG	33.3	50.0	54.0	58.0	913 bp	1.0	41.8
ycf1-1_p2	ACTGGTTTTTCTATTTTCGCT	CATTGGCTCAATGGGGAAGA	33.3	50.0	54.0	57.9	799 bp	1.0	41.8
ycf1-1_p3	AACTGGTTTTTCTATTTTCGCT	TAACGTTTCGTGCTAGTCCG	31.8	50.0	54.8	58.0	914 bp	1.0	41.8
ycf1-1_p4	ACTGGTTTTTCTATTTTCGCTT	TAACGTTTCGTGCTAGTCCG	31.8	50.0	54.8	58.0	913 bp	1.0	41.8
ycf1-1_p5	ACTGGTTTTTCTATTTTCGCT	AAAAACAAAAGCAGACCGCC	33.3	45.0	54.0	58.1	846 bp	1.0	41.7