Hotspot

rps14

Genome · 42430-42732 · 303 bp

Back to Frullania

Pi 0.0099

MarkerSeek score 43.60

Primer available yes

Label rps14

Feature Metadata

label_name: rps14
feature_type: gene
region: Genome
length_bp: 303
variable_sites: 3
pi: 0.0099
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 43.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14_p1	AAGGTTATGAGCCTTGCGAG	CTCACGAGCGTACTCCTCTA	50.0	55.0	58.0	58.1	641-643 bp	1.0	81.9
rps14_p2	AAGGTTATGAGCCTTGCGAG	TCACGAGCGTACTCCTCTAG	50.0	55.0	58.0	58.1	640-642 bp	1.0	81.9
rps14_p3	CGTTATGGATCGGGTTGGTT	CTCACGAGCGTACTCCTCTA	50.0	55.0	58.0	58.1	1023-1034 bp	1.0	81.5
rps14_p4	CGTTATGGATCGGGTTGGTT	TCACGAGCGTACTCCTCTAG	50.0	55.0	58.0	58.1	1022-1033 bp	1.0	81.5
rps14_p5	TACCAGACTGCTCTACTCCG	TCACGAGCGTACTCCTCTAG	55.0	55.0	58.0	58.1	621 bp	0.5	59.2