Hotspot

rpl32-trnP-GGG

Genome · 94407-95201 · 795 bp

Back to Frullania

Pi 0.0303
MarkerSeek score 62.70
Primer available yes
Label rpl32-trnP-GGG

Feature Metadata

label_name
rpl32-trnP-GGG
feature_type
igs
region
Genome
length_bp
795
variable_sites
24
pi
0.0303
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnP-GGG_p1 CAACTTCGAGTAGAACCGGG ATTGCGCTAGGAGCAGTTAC 55.0 50.0 58.0 58.1 2164-2197 bp 1.0 81.3
rpl32-trnP-GGG_p2 CAACTTCGAGTAGAACCGGG CATTGCGCTAGGAGCAGTTA 55.0 50.0 58.0 58.1 2165-2198 bp 1.0 81.3
rpl32-trnP-GGG_p3 CAACTTCGAGTAGAACCGGG CTAACATTGCGCTAGGAGCA 55.0 50.0 58.0 58.1 2169-2202 bp 1.0 81.3
rpl32-trnP-GGG_p4 CAACTTCGAGTAGAACCGGG TAACATTGCGCTAGGAGCAG 55.0 50.0 58.0 58.1 2168-2201 bp 1.0 81.3
rpl32-trnP-GGG_p5 ACACGACGAAAATTGGGACA ATTGCGCTAGGAGCAGTTAC 45.0 50.0 58.0 58.1 1930-1963 bp 1.0 81.1