Hotspot

rpl2-rpl23

Genome · 81612-81642 · 31 bp

Back to Frullania

Pi 0.129

MarkerSeek score 73.80

Primer available yes

Label rpl2-rpl23

Feature Metadata

label_name: rpl2-rpl23
feature_type: igs
region: Genome
length_bp: 31
variable_sites: 4
pi: 0.129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2-rpl23_p1	TTCGGTAAAGGCGTTTGTGA	AATTGGCGAACTCACAGGTT	45.0	45.0	58.0	58.0	672-691 bp	1.0	81.1
rpl2-rpl23_p2	GTTGCGTGTACCTGGTGTAT	AATTGGCGAACTCACAGGTT	50.0	45.0	57.9	58.0	527-546 bp	1.0	80.8
rpl2-rpl23_p3	TAAAGGCGTTTGTGACCTCC	AATTGGCGAACTCACAGGTT	50.0	45.0	58.1	58.0	667-686 bp	1.0	80.6
rpl2-rpl23_p4	ACCTGGTGTATAAGCTCGGT	AATTGGCGAACTCACAGGTT	50.0	45.0	58.1	58.0	518-537 bp	1.0	80.4
rpl2-rpl23_p5	TACAGATCGGTTGCGTGTAC	AATTGGCGAACTCACAGGTT	50.0	45.0	57.7	58.0	536-555 bp	1.0	79.3