Hotspot

rpl22-rps19

Genome · 79862-79878 · 17 bp

Back to Frullania

Pi 0.0588

MarkerSeek score 71.40

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: Genome
length_bp: 17
variable_sites: 1
pi: 0.0588
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	GCTTTGCGACAGCTTTGATT	CGACACGAACTTTTAAAGGGC	45.0	47.6	57.9	58.1	116 bp	1.0	71.5
rpl22-rps19_p2	GCTTTGCGACAGCTTTGATT	CGAACTTTTAAAGGGCATGCA	45.0	42.9	57.9	57.7	111 bp	1.0	69.9
rpl22-rps19_p3	GCTTTGCGACAGCTTTGATT	ACGAACTTTTAAAGGGCATGC	45.0	42.9	57.9	57.7	112 bp	1.0	69.9
rpl22-rps19_p4	CGTATATGCTTTGCGACAGC	CGACACGAACTTTTAAAGGGC	50.0	47.6	57.7	58.1	123 bp	1.0	69.7
rpl22-rps19_p5	CGTATATGCTTTGCGACAGC	CGAACTTTTAAAGGGCATGCA	50.0	42.9	57.7	57.7	118 bp	1.0	68.0