Hotspot

petA-psbJ

Genome · 63359-63501 · 143 bp

Back to Frullania

Pi 0.035

MarkerSeek score 70.00

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: Genome
length_bp: 143
variable_sites: 5
pi: 0.035
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TTTTTCGGGTCGGTTGTTCT	ATCGTCGTGATTGGTTTGGT	45.0	45.0	58.0	57.8	302 bp	1.0	79.6
petA-psbJ_p2	TTTTTCGGGTCGGTTGTTCT	ATTGGTACGGGAGCTGGTAT	45.0	50.0	58.0	58.2	320 bp	1.0	79.3
petA-psbJ_p3	TTTTTCGGGTCGGTTGTTCT	AGCTGGTATCGTCGTGATTG	45.0	50.0	58.0	57.8	309 bp	1.0	79.3
petA-psbJ_p4	TTTTTCGGGTCGGTTGTTCT	CTAATTGGTACGGGAGCTGG	45.0	55.0	58.0	57.8	323 bp	1.0	79.0
petA-psbJ_p5	TCGGTTGTTCTAGCGCAAAT	ATCGTCGTGATTGGTTTGGT	45.0	45.0	58.2	57.8	293 bp	1.0	78.4