Hotspot

cemA-petA

Genome · 62194-62401 · 208 bp

Back to Frullania

Pi 0.0493

MarkerSeek score 74.30

Primer available yes

Label cemA-petA

Feature Metadata

label_name: cemA-petA
feature_type: igs
region: Genome
length_bp: 208
variable_sites: 10
pi: 0.0493
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA-petA_p1	ACCTTCCATTGTAGCAACGT	CGAAAATCGATCGAGTTACCC	45.0	47.6	57.4	56.9	306-310 bp	1.0	62.6
cemA-petA_p2	CACCTTCCATTGTAGCAACG	CGAAAATCGATCGAGTTACCC	50.0	47.6	57.1	56.9	307-311 bp	1.0	59.9
cemA-petA_p3	ACCTTCCATTGTAGCAACGT	ACGAAAATCGATCGAGTTACCC	45.0	45.5	57.4	58.6	307-311 bp	1.0	58.9
cemA-petA_p4	ACCTTCCATTGTAGCAACGT	CGAAAATCGATCGAGTTACCCA	45.0	45.5	57.4	58.6	306-310 bp	1.0	58.9
cemA-petA_p5	ACCTTCCATTGTAGCAACGT	ACGAAAATCGATCGAGTTACC	45.0	42.9	57.4	56.2	307-311 bp	1.0	57.0