Hotspot

trnM-CAU-rps14

Genome · 42337-42429 · 93 bp

Back to Frullania

Pi 0.129

MarkerSeek score 72.90

Primer available yes

Label trnM-CAU-rps14

Feature Metadata

label_name: trnM-CAU-rps14
feature_type: igs
region: Genome
length_bp: 93
variable_sites: 12
pi: 0.129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-rps14_p1	AAGGTTATGAGCCTTGCGAG	CAGTGCACCTAGTCGTCTTC	50.0	55.0	58.0	58.0	281 bp	1.0	83.6
trnM-CAU-rps14_p2	AAGGTTATGAGCCTTGCGAG	AGTCGTCTTCATCGTCGTTG	50.0	50.0	58.0	58.0	271 bp	1.0	83.3
trnM-CAU-rps14_p3	CGTTATGGATCGGGTTGGTT	CAGTGCACCTAGTCGTCTTC	50.0	55.0	58.0	58.0	661-674 bp	1.0	82.2
trnM-CAU-rps14_p4	GCTTCGTTATGGATCGGGTT	CAGTGCACCTAGTCGTCTTC	50.0	55.0	58.1	58.0	665-678 bp	1.0	82.1
trnM-CAU-rps14_p5	TACCAGACTGCTCTACTCCG	CAGTGCACCTAGTCGTCTTC	55.0	55.0	58.0	58.0	260 bp	0.5	59.6