Hotspot

trnE-UUC-trnT-GGU

Genome · 37526-38342 · 817 bp

Back to Frullania

Pi 0.0327

MarkerSeek score 69.30

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: Genome
length_bp: 817
variable_sites: 26
pi: 0.0327
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	GTCGCCTCCTTGAAAGAGAG	GAACCGATGACTTACGCCTT	55.0	50.0	58.0	58.0	920-925 bp	1.0	84.2
trnE-UUC-trnT-GGU_p2	GTCGCCTCCTTGAAAGAGAG	CCATGGCGTTACTCTACCAC	55.0	55.0	58.0	58.1	899-904 bp	1.0	83.8
trnE-UUC-trnT-GGU_p3	TCGCCTCCTTGAAAGAGAGA	GAACCGATGACTTACGCCTT	50.0	50.0	58.1	58.0	919-924 bp	1.0	83.6
trnE-UUC-trnT-GGU_p4	GTCGCCTCCTTGAAAGAGAG	TGGCGTTACTCTACCACTGA	55.0	50.0	58.0	58.1	896-901 bp	1.0	83.6
trnE-UUC-trnT-GGU_p5	TCGCCTCCTTGAAAGAGAGA	CCATGGCGTTACTCTACCAC	50.0	55.0	58.1	58.1	898-903 bp	1.0	83.2