Hotspot

rps7-ndhB

Genome · 1542-1724 · 183 bp

Back to Frullania

Pi 0.0391

MarkerSeek score 70.10

Primer available yes

Label rps7-ndhB

Feature Metadata

label_name: rps7-ndhB
feature_type: igs
region: Genome
length_bp: 183
variable_sites: 7
pi: 0.0391
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps7-ndhB_p1	TGAACTGATAGATGCAGCCA	AAAAAGGATGTACCAAGTTCT	45.0	33.3	56.6	52.9	315-316 bp	1.0	42.8
rps7-ndhB_p2	TGAACTGATAGATGCAGCCA	AGGATGTACCAAGTTCTAATTTCA	45.0	33.3	56.6	55.8	311-312 bp	1.0	42.8
rps7-ndhB_p3	TGAACTGATAGATGCAGCCAA	AAAAAGGATGTACCAAGTTCT	42.9	33.3	57.4	52.9	315-316 bp	1.0	42.8
rps7-ndhB_p4	TGAACTGATAGATGCAGCCAA	AGGATGTACCAAGTTCTAATTTCA	42.9	33.3	57.4	55.8	311-312 bp	1.0	42.8
rps7-ndhB_p5	TGAACTGATAGATGCAGCCA	AAGGATGTACCAAGTTCTAATTTCA	45.0	32.0	56.6	56.4	312-313 bp	1.0	42.8