Hotspot

ycf1

SSC · 121515-127058 · 5544 bp

Back to Fritillaria

Pi 0.0126

MarkerSeek score 56.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5544
variable_sites: 421
pi: 0.0126
unique_haplotype_count: 31
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGTATGGTCCCTTCATGGT	TTGTCTGAAACCTTGGCGAA	50.0	45.0	58.0	58.0	436-448 bp	0.787879	80.1
ycf1_p2	TGGTATGGTCCCTTCATGGT	TTTGTCTGAAACCTTGGCGA	50.0	45.0	58.0	58.0	437-449 bp	0.787879	80.1
ycf1_p3	TGGTATGGTCCCTTCATGGT	TCTGAAACCTTGGCGAAGAC	50.0	50.0	58.0	58.1	433-445 bp	0.727273	77.1
ycf1_p4	TGGTATGGTCCCTTCATGGT	AGAAGGGGCAAAAGAACAGG	50.0	50.0	58.0	58.0	775-781 bp	0.484848	64.1
ycf1_p5	TGGTATGGTCCCTTCATGGT	GAAGGGGCAAAAGAACAGGA	50.0	50.0	58.0	58.0	774-780 bp	0.484848	64.0