Hotspot

rpl22-rps19

LSC · 81536-81643 · 108 bp

Back to Fritillaria

Pi 0.0409

MarkerSeek score 70.60

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 108
variable_sites: 26
pi: 0.0409
unique_haplotype_count: 26
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	TTTCTGTACTCCAACGCGAG	CAAGACATGCAAAAACCGAT	50.0	40.0	57.9	55.2	175-222 bp	0.969697	67.6
rpl22-rps19_p2	GCGTCCACGAATCTGATCAA	CAAGACATGCAAAAACCGAT	50.0	40.0	58.4	55.2	255-302 bp	0.969697	65.2
rpl22-rps19_p3	CGTGCTTTGAAAACCGACAT	CAAGACATGCAAAAACCGAT	45.0	40.0	57.6	55.2	227-274 bp	0.969697	64.9
rpl22-rps19_p4	GAGCGTCCACGAATCTGATC	CAAGACATGCAAAAACCGAT	55.0	40.0	58.5	55.2	257-304 bp	0.969697	63.9
rpl22-rps19_p5	TTCGTGCTTTGAAAACCGAC	CAAGACATGCAAAAACCGAT	45.0	40.0	57.5	55.2	229-276 bp	0.939394	63.2