Hotspot

psbE-petL

LSC · 62202-63417 · 1216 bp

Back to Fritillaria

Pi 0.0105

MarkerSeek score 70.20

Primer available yes

Label psbE-petL

Feature Metadata

label_name: psbE-petL
feature_type: igs
region: LSC
length_bp: 1216
variable_sites: 88
pi: 0.0105
unique_haplotype_count: 32
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 70.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbE-petL_p1	ACTGACGAATAACCAACCCG	ACCAATCAATAGAGCTGAGGT	50.0	42.9	57.9	56.4	1349-1406 bp	1.0	67.9
psbE-petL_p2	ACGAATAACCAACCCGCAAT	ACCAATCAATAGAGCTGAGGT	45.0	42.9	58.2	56.4	1345-1402 bp	1.0	67.2
psbE-petL_p3	CCCGCAATAAATAGGGAGGG	ACCAATCAATAGAGCTGAGGT	55.0	42.9	57.8	56.4	1333-1390 bp	1.0	67.0
psbE-petL_p4	ACTGACGAATAACCAACCCG	AGCTGAGGTTATAGTTGAAGCA	50.0	40.9	57.9	57.2	1337-1394 bp	1.0	66.0
psbE-petL_p5	ACTGACGAATAACCAACCCG	TGAGGTTATAGTTGAAGCAGCT	50.0	40.9	57.9	57.2	1334-1391 bp	1.0	66.0