Hotspot

petA-psbJ

LSC · 60624-61427 · 804 bp

Back to Fritillaria

Pi 0.0154

MarkerSeek score 66.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 804
variable_sites: 74
pi: 0.0154
unique_haplotype_count: 25
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CATTACGTGTCCAAGGCCTT	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.2	58.3	946-967 bp	1.0	83.4
petA-psbJ_p2	CATTACGTGTCCAAGGCCTT	CATATTCCGGGTTGGGTTCA	50.0	50.0	58.2	57.6	942-963 bp	1.0	82.5
petA-psbJ_p3	CATTACGTGTCCAAGGCCTT	GTTCATATTCCGGGTTGGGT	50.0	50.0	58.2	57.6	945-966 bp	1.0	82.5
petA-psbJ_p4	TTACGTGTCCAAGGCCTTTT	GGTTCATATTCCGGGTTGGG	45.0	55.0	57.6	58.3	944-965 bp	1.0	82.1
petA-psbJ_p5	TACGTGTCCAAGGCCTTTTG	GGTTCATATTCCGGGTTGGG	50.0	55.0	58.4	58.3	943-964 bp	1.0	81.7