Hotspot

trnT-GGU-psbD

LSC · 29716-30824 · 1109 bp

Back to Fritillaria

Pi 0.0138

MarkerSeek score 63.90

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1109
variable_sites: 90
pi: 0.0138
unique_haplotype_count: 30
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	TCTACCAAGGGCTACAGTCA	55.0	50.0	58.1	57.4	974-1192 bp	1.0	84.9
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	TCTACCAAGGGCTACAGTCA	50.0	50.0	58.1	57.4	977-1195 bp	1.0	84.7
trnT-GGU-psbD_p3	GGCGTAAGTCATCGGTTCAA	TCTACCAAGGGCTACAGTCA	50.0	50.0	58.3	57.4	951-1169 bp	1.0	83.2
trnT-GGU-psbD_p4	GGCCCTTTTAACTCAGTGGT	TCTACCAAGGGCTACAGTCA	50.0	50.0	57.7	57.4	989-1207 bp	1.0	83.0
trnT-GGU-psbD_p5	TGGTAGAGTAACGCCATGGT	TCTACCAAGGGCTACAGTCA	50.0	50.0	58.4	57.4	973-1191 bp	1.0	81.9