Hotspot

psbM-trnD-GUC

LSC · 27317-28374 · 1058 bp

Back to Fritillaria

Pi 0.0153

MarkerSeek score 66.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1058
variable_sites: 107
pi: 0.0153
unique_haplotype_count: 29
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TGCGAGAATATTAACTTCCA	CCTCGCTGGGATTGTAGTTC	35.0	55.0	52.0	58.1	1101-1160 bp	1.0	51.7
psbM-trnD-GUC_p2	TGCGAGAATATTAACTTCCA	TACACCTCGCTGGGATTGTA	35.0	50.0	52.0	58.1	1105-1164 bp	1.0	51.7
psbM-trnD-GUC_p3	ATGCGAGAATATTAACTTCCA	CCTCGCTGGGATTGTAGTTC	33.3	55.0	52.7	58.1	1102-1161 bp	1.0	51.7
psbM-trnD-GUC_p4	TGCGAGAATATTAACTTCCAT	CCTCGCTGGGATTGTAGTTC	33.3	55.0	52.7	58.1	1101-1160 bp	1.0	51.7
psbM-trnD-GUC_p5	TGCGAGAATATTAACTTCCA	CATACACCTCGCTGGGATTG	35.0	55.0	52.0	58.4	1107-1166 bp	1.0	51.7