Hotspot

rpoB-trnC-GCA

LSC · 25823-26493 · 671 bp

Back to Fritillaria

Pi 0.017

MarkerSeek score 72.00

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 671
variable_sites: 74
pi: 0.017
unique_haplotype_count: 28
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 72.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AAGCCCCGATTAATGAACCC	GCGACACCCAGATTTGAACT	50.0	50.0	57.9	58.5	811-834 bp	1.0	87.3
rpoB-trnC-GCA_p2	AAGCCCCGATTAATGAACCC	CCTTACCACTTGGCCATGTC	50.0	55.0	57.9	58.5	765-788 bp	1.0	87.0
rpoB-trnC-GCA_p3	AAGCCCCGATTAATGAACCC	TTTGTCAATCAGGCGACACC	50.0	50.0	57.9	58.8	823-846 bp	1.0	84.9
rpoB-trnC-GCA_p4	CCCCGATTAATGAACCCACA	GCGACACCCAGATTTGAACT	50.0	50.0	57.6	58.5	808-831 bp	1.0	84.5
rpoB-trnC-GCA_p5	AAGCCCCGATTAATGAACCC	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.9	57.5	786-809 bp	0.939394	84.0