Hotspot

rpl32-trnL(UAG)

SSC · 114918-115779 · 862 bp

Back to Fraxinus

Pi 0.0102
MarkerSeek score 57.30
Primer available yes
Label rpl32-trnL(UAG)

Feature Metadata

label_name
rpl32-trnL(UAG)
feature_type
igs
region
SSC
length_bp
862
variable_sites
38
pi
0.0102
unique_haplotype_count
18
primer_available
yes
alignment_reliability
0.99
markerseek_score
57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL(UAG)_p1 TGGGACAGCGTTAAAAGCAT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 983-1001 bp 1.0 83.5
rpl32-trnL(UAG)_p2 TGGGACAGCGTTAAAAGCAT AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 976-994 bp 1.0 83.1
rpl32-trnL(UAG)_p3 TGGGACAGCGTTAAAAGCAT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 58.1 57.8 982-1000 bp 1.0 82.9
rpl32-trnL(UAG)_p4 TGGGACAGCGTTAAAAGCAT CTAAGAGCAGCGTGTCTACC 45.0 55.0 58.1 57.8 981-999 bp 1.0 82.9
rpl32-trnL(UAG)_p5 TGGGACAGCGTTAAAAGCAT GAGCAGCGTGTCTACCAATT 45.0 50.0 58.1 58.3 977-995 bp 1.0 82.3