Hotspot

ndhF-rpl32

SSC · 114328-114755 · 428 bp

Back to Fraxinus

Pi 0.0168

MarkerSeek score 53.50

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 428
variable_sites: 33
pi: 0.0168
unique_haplotype_count: 17
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GTTGCCGTCGGAAAAAGAAG	ACGCGTTTTTGATGCAGAAG	50.0	45.0	58.0	58.0	566-623 bp	1.0	84.9
ndhF-rpl32_p2	GTTGCCGTCGGAAAAAGAAG	CGCGTTTTTGATGCAGAAGT	50.0	45.0	58.0	58.0	565-622 bp	1.0	84.9
ndhF-rpl32_p3	TGCCGTCGGAAAAAGAAGAA	ACGCGTTTTTGATGCAGAAG	45.0	45.0	58.0	58.0	564-621 bp	1.0	84.8
ndhF-rpl32_p4	TGCCGTCGGAAAAAGAAGAA	CGCGTTTTTGATGCAGAAGT	45.0	45.0	58.0	58.0	563-620 bp	1.0	84.8
ndhF-rpl32_p5	TTGCCGTCGGAAAAAGAAGA	CGCGTTTTTGATGCAGAAGT	45.0	45.0	58.0	58.0	564-621 bp	1.0	84.8