Hotspot

rpl22-rps19

LSC · 86086-86150 · 65 bp

Back to Fraxinus

Pi 0.0282
MarkerSeek score 65.10
Primer available yes
Label rpl22-rps19

Feature Metadata

label_name
rpl22-rps19
feature_type
igs
region
LSC
length_bp
65
variable_sites
6
pi
0.0282
unique_haplotype_count
13
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl22-rps19_p1 AAGAGCGTCCACGAATTTGA AGGACACAAATTGGGCGAAT 45.0 45.0 57.8 58.1 247 bp 0.041667 39.7
rpl22-rps19_p2 AAGAGCGTCCACGAATTTGA TGGTAGGACACAAATTGGGC 45.0 50.0 57.8 58.1 251 bp 0.041667 39.7
rpl22-rps19_p3 AAGAGCGTCCACGAATTTGA GTAGGACACAAATTGGGCGA 45.0 50.0 57.8 58.2 249 bp 0.041667 38.9
rpl22-rps19_p4 AGAGCGTCCACGAATTTGAT AGGACACAAATTGGGCGAAT 45.0 45.0 57.6 58.1 246 bp 0.041667 38.0
rpl22-rps19_p5 AGAGCGTCCACGAATTTGAT TGGTAGGACACAAATTGGGC 45.0 50.0 57.6 58.1 250 bp 0.041667 37.9