Hotspot

trnF(GAA)-ndhJ

LSC · 50033-50700 · 668 bp

Back to Fraxinus

Pi 0.00527

MarkerSeek score 52.40

Primer available yes

Label trnF(GAA)-ndhJ

Feature Metadata

label_name: trnF(GAA)-ndhJ
feature_type: igs
region: LSC
length_bp: 668
variable_sites: 16
pi: 0.00527
unique_haplotype_count: 13
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF(GAA)-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	811-815 bp	1.0	81.1
trnF(GAA)-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	775-779 bp	1.0	80.7
trnF(GAA)-ndhJ_p3	TCAAATCTGGTTCCTGGCAC	AGTTGGATAGGATGGCCCTT	50.0	50.0	58.1	58.1	760-764 bp	1.0	80.5
trnF(GAA)-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	ATCATCCACGCTTGAAACGT	55.0	45.0	58.0	58.2	846-850 bp	1.0	80.2
trnF(GAA)-ndhJ_p5	CTGGTAGAGCAGAGGACTGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	57.9	58.1	799-803 bp	1.0	80.0