Hotspot

atpA-atpF

LSC · 12701-12770 · 70 bp

Back to Fraxinus

Pi 0.0393

MarkerSeek score 67.10

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 70
variable_sites: 7
pi: 0.0393
unique_haplotype_count: 16
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ACTAATTTCGTCGGCTCGAA	TGTTGGGAGCGATGAAAGAA	45.0	45.0	57.4	57.4	124-134 bp	1.0	78.8
atpA-atpF_p2	ACTAATTTCGTCGGCTCGAA	ATATTGGCATGTTGGGAGCG	45.0	50.0	57.4	58.7	133-143 bp	1.0	77.5
atpA-atpF_p3	ACTAATTTCGTCGGCTCGAA	ATGTTGGGAGCGATGAAAGA	45.0	45.0	57.4	57.2	125-135 bp	1.0	76.9
atpA-atpF_p4	ACTAATTTCGTCGGCTCGAA	CATGTTGGGAGCGATGAAAG	45.0	50.0	57.4	57.2	126-136 bp	1.0	76.5
atpA-atpF_p5	AATTTCGTCGGCTCGAATGG	TGTTGGGAGCGATGAAAGAA	50.0	45.0	59.0	57.4	121-131 bp	1.0	76.1