Hotspot

trnH(GUG)-psbA

LSC · 94-530 · 437 bp

Back to Fraxinus

Pi 0.0322
MarkerSeek score 67.40
Primer available yes
Label trnH(GUG)-psbA

Feature Metadata

label_name
trnH(GUG)-psbA
feature_type
igs
region
LSC
length_bp
437
variable_sites
47
pi
0.0322
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GUG)-psbA_p1 CTGCCTTAATCCACTTGGCT CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 508-574 bp 1.0 87.0
trnH(GUG)-psbA_p2 ACTGCCTTAATCCACTTGGC CCGTGCTAACCTTGGTATGG 50.0 55.0 58.2 58.3 509-575 bp 1.0 86.8
trnH(GUG)-psbA_p3 CTGCCTTAATCCACTTGGCT ACCGTGCTAACCTTGGTATG 50.0 50.0 57.9 57.6 509-575 bp 1.0 86.5
trnH(GUG)-psbA_p4 ACTGCCTTAATCCACTTGGC ACCGTGCTAACCTTGGTATG 50.0 50.0 58.2 57.6 510-576 bp 1.0 86.3
trnH(GUG)-psbA_p5 AATCCACTTGGCTACATCCG CCGTGCTAACCTTGGTATGG 50.0 55.0 57.7 58.3 501-567 bp 1.0 85.5