Hotspot

ycf1

SSC · 123441-128942 · 5502 bp

Back to Frankenia

Pi 0.0129

MarkerSeek score 48.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5502
variable_sites: 71
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACGGATTAATCGAGCAGTTCT	GAATTTCCTTTTGGCTCGCC	42.9	50.0	57.2	58.0	525 bp	1.0	67.7
ycf1_p2	AGGCTTGTGAGAGAAAGGAT	GAATTTCCTTTTGGCTCGCC	45.0	50.0	56.1	58.0	826 bp	1.0	66.7
ycf1_p3	ACGGATTAATCGAGCAGTTCT	AAAGGCGTGGAATCGAGAAA	42.9	45.0	57.2	57.8	757 bp	1.0	65.8
ycf1_p4	AGGCTTGTGAGAGAAAGGAT	AAAGGCGTGGAATCGAGAAA	45.0	45.0	56.1	57.8	1058 bp	1.0	65.1
ycf1_p5	ACGGATTAATCGAGCAGTTCT	CGAAATTGCAGAAACGTGGG	42.9	50.0	57.2	58.3	925 bp	1.0	65.0